An Agarose-Gel Based Method for Transporting Cell Lines

An Agarose-Gel Based Method for Transporting Cell Lines

Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times...

Calibration of an alanine/agarose gel

Assessing neural stem cell motility using an agarose gel-based microfluidic device.

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage. This is largely due to the difficulty with which one can create a cell-compatible and steady microenvironment using conventional microfabrication techniques and materials. We address this problem via the introduction of a novel microfabrication material, a...

Agarose gel electrophoresis.

MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...

Agarose gel electrophoresis.

Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.